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Overview of Image Analysis Programs for Biofilm Confocal Micrographs with a Focus on Tool Scope and Selection of Relevance to Experimental Researchers <xref ref-type= a " width="100%" height="100%">

Journal: ACS Omega

Article Title: Tools of the Trade: Image Analysis Programs for Confocal Laser-Scanning Microscopy Studies of Biofilms and Considerations for Their Use by Experimental Researchers

doi: 10.1021/acsomega.2c07255

Figure Lengend Snippet: Overview of Image Analysis Programs for Biofilm Confocal Micrographs with a Focus on Tool Scope and Selection of Relevance to Experimental Researchers a

Article Snippet: PHLIP , free, open-access, available at https://sourceforge.net/projects/phlip/ , Windows, Linux, macOS , MATLAB toolbox-based script , GUI , segmentation; biofilm volume, morphology, and surface characterization , allows batch processing; offers both manual and automated (Otsu’s Method) thresholding; multichannel analysis; biofilm spatial and temporal calculations: biovolume, substratum coverage, thickness, roughness and colocalization; offers a data conversion option (PHLIP-ML) to convert different image formats to input format; has direct connectivity to PHLIP-ML (file convertor) for Leica Systems , MATLAB-based and requires MATLAB licensing; requires preprocessing steps such as image inversion and x-section resolution.

Techniques: Selection, Fluorescence, Bacteria, Staining, Cytometry, Labeling, Cell Counting, Introduce, Filtration, Activity Assay, Software, Microscopy